Recently, we have been applying a library preparation technique from paleogenomics to cell-free DNA (cfDNA) studies. We found that such a method (adapting all cfDNA as single-strands rather than double-strands) allows us to collect forms that are more degraded in blood. Our analysis has lead to much higher capture rates of microbial and mitochondrial cell-free DNA, which we apply to the case of lung transplant patients. Other groups have applied the technique to create better tests for fetal abnormalities and to determine tissue-specific origins of the cfDNA. Check out our group's first paper in Scientific Reports. We recently applied an extension of this method to determine the efficacy of utilizing cell-free DNA as a replacement for urine culture, urinalysis, and viruria qPCR detection.
Improving blood microbiome studies
Physical analysis of nanoparticles for therapeutics